Gasi chromatograph jekiseni tsonokazhinji shandisa 1ul uye 10ul. Kunyangwe tsono yejekiseni iri diki, yakakosha. Iyo jekiseni tsono ndiyo chiteshi inobatanidza sampuli uye analytical chiridzwa. Iine jekiseni tsono, sampuli inogona kupinda chromatographic column uye kupfuura detector kuti inoenderera spectrum kuongororwa. Naizvozvo, kuchengetedza uye kucheneswa kwejekiseni tsono ndiyo inotariswa nevanoongorora zuva nezuva. Kana zvisina kudaro, hazvizongokanganisa kushanda kwekushanda, asiwo zvinokonzera kukanganisa kune chiridzwa. Mufananidzo unotevera unoratidza zvikamu zvejekiseni tsono.
Kurongeka kwemajekiseni tsono
Zvinoenderana nekuonekwa kwejekiseni rejekiseni, rinogona kupatsanurwa kuita conical jekiseni tsono, bevel jekiseni tsono, uye flat-head jekiseni tsono. Conical tsono dzinoshandiswa septum jekiseni, izvo zvinogona kuderedza kukuvadzwa kweseptum uye kutsungirira majekiseni akawanda. Iwo anonyanya kushandiswa mune otomatiki majekiseni; bevel tsono dzinogona kushandiswa pane jekiseni septa, iri nyore kushanda. Pakati pavo, 26s-22 tsono inonyanya kukodzera kushandiswa pane jekiseni septa mugasi chromatography; jekiseni remusoro-soro rinonyanya kushandiswa pamajekiseni mavharuvhu uye emuenzaniso pipettes epamusoro-inoshanda liquid chromatographs.
Zvinoenderana nejekiseni nzira, inogona kupatsanurwa kuita otomatiki jekiseni tsono uye manual jekiseni tsono.
Zvinoenderana neakasiyana ekuongorora zvinodiwa zvejekiseni tsono mune gasi chromatograph uye liquid chromatograph fluid, inogona kupatsanurwa kuita gasi jekiseni tsono uye yemvura jekiseni tsono. Iyo gasi chromatography jekiseni tsono kazhinji inoda shoma jekiseni, uye iro rinowanzozivikanwa jekiseni vhoriyamu i0.2-1ul, saka rinoenderana jekiseni tsono kazhinji 10-25ul. Iyo tsono yakasarudzwa ndeye cone mhando tsono, iyo yakanakira jekiseni kushanda; mukuenzanisa, iyo yemvura chromatography jekiseni vhoriyamu kazhinji yakakura, uye yakajairika jekiseni vhoriyamu inosvika 0.5-20ul, saka vhoriyamu yetsono yakakura zvakare, kazhinji 25-100UL, uye tsono yetsono yakati sandara kudzivirira kukwenya stator.
Mukuongorora chromatographic, iyo inonyanya kushandiswa jekiseni tsono idiki jekiseni tsono, iyo inonyanya kukodzera gasi chromatograph uye liquid chromatograph liquid analysis. Kukanganisa kwayo kwese kwesimba ndeye ± 5%. Kuita kwemhepo kunomira 0.2Mpa. Yakakamurwa kuva mhando mbiri: mvura yekuchengetedza injector uye mvura yekuchengetedza injector. Iyo yemhando yemhando yeiyo isiri-mvura diki-injector ndeye 0.5μL-5μL, uye yakatarwa yemhando yemvura micro-injector ndeye 10μL-100μL. Iyo micro-jekiseni tsono chinhu chakakosha chechokwadi chiridzwa.
Kushandisa jekiseni
(1) Tarisa jekiseni usati washandisa, tarisa kana sirinji ine makatsemuka uye kana tsono yetsono yakatsva.
(2) Bvisa sampu yakasara mujekitori, geza jekiseni ne solvent 5 ~ 20 nguva, uye urase tsvina yemvura kubva pakutanga 2 ~ 3 nguva.
(3) Bvisa mabubble mujekiseni, nyudza tsono musolvent, uye dzokorora dhizaini sampuli. Paunenge uchidonhedza sampuli, mabhubhu ari mujekiseni anogona kuchinja nekushanduka kwakamira kwechubhu.
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Kugadziriswa kwetsono yejekiseni
(1) Pakati nepamusoro viscosity samples dzinofanira kuderedzwa kana hombe yemukati dhayamita jekiseni tsono inofanira kusarudzwa isati yashandiswa.
(2) Pakuchenesa tsono, maturusi okuchenesa anofanira kushandiswa, akadai sewaya yekutungamira kana kuti stylet, ma tweezers, uye mafizita anofanira kushandiswa kuchenesa madziro etsono.
(3) Kucheneswa kwekupisa: Kucheneswa kwemafuta kunoshandiswa kubvisa organic residues pane tsono, kunyanya kuongororwa kwekutsvaga, nzvimbo yekubikira yakakwirira uye zvinhu zvinonamira. Mushure memaminitsi mashoma ekuchenesa kupisa, tsono yekuchenesa mudziyo inogona kushandiswa zvakare.
Kucheneswa kwejekiseni tsono
1. Rusvingo rwemukati rwejojo jekiseni rinogona kucheneswa ne organic solvent. Paunenge uchichenesa, ndapota tarisa kana jekiseni tsono kusunda tsvimbo inogona kufamba zvakanaka;
2. Kana jekiseni tsono kusunda tsvimbo isingafambi zvakanaka, tsvimbo yekusundidzira inogona kubviswa. Inokurudzirwa kuipukuta yakachena nemucheka munyoro wakanyikwa mu organic solvent.
3. Dzokorora shandisa organic solvent kushuvira. Kana kushorwa kwejekiseni rekusimudzira tsono kuchiwedzera nekukurumidza mushure mekushuvira kwakawanda, zvinoreva kuti kuchine tsvina diki. Muchiitiko ichi, nzira yekuchenesa inoda kudzokororwa.
4. Kana iyo jekiseni inopinza tsvimbo inogona kufamba zvakanaka uye zvishoma nezvishoma, tarisa kana tsono yakavharwa. Dzokorora sukurudza tsono neorganic solvent uye tarisa chimiro chemuenzaniso uri kusundirwa kunze kwetsono.
5. Kana tsono yejekiseni iri yakajairika, sampuli inoyerera ichibuda mumutsara wakatwasuka. Kana tsono yakavharwa, sample inopfapfaidzwa mumhute yakanaka kubva kune imwe nzira kana kona. Kunyangwe kana iyo solvent dzimwe nguva ichiyerera ichibuda mumutsara wakatwasuka, chenjerera kutarisa kuti kuyerera kuri nani pane zvakajairika (ingoenzanisa kuyerera neitsva, isina kuvharwa jekiseni tsono).
6. Kuvhara mutsono kuchaparadza kuberekazve kwekuongorora. Nokuda kwechikonzero ichi, kugadziriswa kwetsono kunodiwa. Shandisa chinhu chakaita sewaya kubvisa kuvharika mutsono. Iyo tsono inogona kushandiswa chete kana sampuli ichiyerera kunze kwakajairika. Kushandisa pipette kushuvira mvura kana sirinji yekuchenesa kunogonawo kubvisa zvinosvibisa mutsono.
Chengetedzo kana uchishandisa jekiseni tsono
Usabate tsono yesirinji nechikamu chemuenzaniso nemaoko ako, uye usave nemafubhu (kana uchishuvira, dhonza zvishoma nezvishoma, nekukurumidza, wozovava zvishoma nezvishoma, dzokorora kakawanda, 10 Huwandu hwetsono yesimbi yesirinji yeμl inosvika 0,6 μl sirinji ine musimboti inonzwa yakapfava) Iyo jekiseni inomhanya inofanira kukurumidza (asi kwete yakanyanya kukurumidza), chengetedza kukurumidza kwakafanana kune imwe neimwe jekiseni, uye tanga kupinza sampuli kana tsono yetsono ichisvika pakati pekamuri yekupisa.
Nzira yekudzivirira sei jekiseni tsono kubva pakukotama? Vazhinji vatsva vanoongorora chromatography vanowanzo kotamisa tsono uye sirinji tsvimbo yesirinji. Zvikonzero ndezvi:
1. Chiteshi chejekiseni chinoputirwa zvakanyanya. Kana yakanyungudutswa zvakanyanya patembiricha yekamuri, chisimbiso chesilicone chichawedzera uye kuomesa kana tembiricha yekamuri yekupisa inokwira. Panguva ino, zvakaoma kuisa sirinji.
2. Iyo tsono inonamira muchikamu chesimbi chejekiseni port kana chinzvimbo chisina kuwanikwa zvakanaka.
3. Sirinji tsvimbo yakakotama nekuti simba rakawandisa rinoshandiswa panguva yejekiseni. Anotyisa, machromatographs anouya nejekiseni rejeki, uye jekiseni nejekiseni rejeki hazvikotamise tsvimbo yesirinji.
4. Nemhaka yokuti rusvingo rwemukati rwesirinji rwakasvibiswa, tsono tsvimbo inosundirwa uye yakakotama panguva yejekiseni. Mushure mekushandisa sirinji kwenguva yakati, uchaona chinhu chidiki chitema padhuze nepamusoro petsono chubhu, uye zvichave zvakaoma kusveta sampuli nekubaya. Nzira yekuchenesa: Dhonza tsono tsvimbo, jekiseni mvura shoma, isa mudonzvo wetsono panzvimbo yakasvibiswa uye sunda nekudhonza kakawanda. Kana ikasashanda kamwe chete, jekiseni mvura zvakare kusvikira tsvina yacho yabviswa. Panguva ino, iwe uchaona kuti mvura iri mujekiseni inoshanduka. Dhonza tsono woipukuta nesefa bepa, wobva wageza nedoro kakawanda. Kana sampuro iongororwe isimbi yakasimba yakanyungudika musolvent, geza sirinji nesolvent munguva mushure mejekiseni.
5. Iva nechokwadi chekugadzikana paunenge uchibaya jekiseni. Kana iwe uchida kukurumidza, sirinji inogonya. Chero bedzi iwe uri nyanzvi mujekiseni, inenge ichikurumidza.
Nguva yekutumira: Jun-19-2024