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Nzira nhanhatu dzekuchenesa HPLC sampuli mabhodhoro

Ndapota ita sarudzo yako pachako zvichienderana nemamiriro erabhoritari yako.

Zvakakosha kuwana nzira inoshanda yekuchenesa mabhodhoro emuenzaniso

Parizvino, kune huwandu hukuru hwezvigadzirwa zvekurima (zvimwe zvigadzirwa zvemakemikari, organic acids, nezvimwewo) zvinoda kuongororwa ne liquid chromatography uye gasi chromatography gore rega rega.Nekuda kwehuwandu hukuru hwemasamples, kune huwandu hukuru hwemasample mabhoti anoda kucheneswa panguva yekuonekwa, izvo zvisingangotambisi nguva uye kuderedza kushanda zvakanaka, asi dzimwe nguva zvinokonzeresa kutsauka mumhedzisiro yekuyedza nekuda kwehutsanana. mabhodhoro emuenzaniso akacheneswa.

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Iyo chromatographic sample vials inonyanya kugadzirwa negirazi, kashoma inogadzirwa nepurasitiki.Mabhodhoro emuenzaniso anoraswa anodhura, anoparadza, uye anosvibisa nharaunda.Marabhoritari mazhinji anochenesa mabhodhoro emuenzaniso uye oashandisa zvakare.

Parizvino, nzira dzinowanzo shandiswa murabhoritari dzekugeza mabhodhoro dzinonyanya kuwedzerwa detergent, detergent, organic solvents uye acid wash, wozogadzika brushing diki chubhu system.

Iyi yakajairika nzira yekukwesha ine zvakawanda zvisingabatsiri:
Kushandiswa kwe detergent kunopedza mvura yakawanda, nguva yekugeza yakareba, uye kune makona asingawanzo kuchenesa.Kana iri mabhodhoro ekuenzanisira epurasitiki, zviri nyore kuve nemavara ebhurashi mukati memadziro evharo, izvo zvinotora zvakawanda zvevashandi.Kune glassware yakasvibiswa zvakanyanya ne lipid uye mapuroteni zvakasara, alkaline lysis solution inoshandiswa pakuchenesa, uye migumisiro yakanaka inowanikwa.

Paunenge uchiongorora samples neLC/MS/MS, kucheneswa kwemajekiseni ejekiseni kwakakosha.Maererano nekuchenesa nzira yegirazi, nzira yekuchenesa inosarudzwa maererano nehuwandu hwekusvibiswa.Iko hakuna yakagadziriswa mode.Nzira pfupiso:

Sarudzo Yekutanga:

1. Dururira mhinduro yekuedza muvharosi yakaoma
2. Dhiraisa mhinduro yose yekuedza mu 95% doro, geza kaviri ne ultrasonic uye uidururire kunze, nokuti doro rinopinda nyore nyore muvharo re 1.5mL uye rinogona kusanganiswa nemafuta akawanda emagetsi kuti awane kushanda kwekuchenesa.
3. Dururira mumvura yakachena, uye ultrasonically geza kaviri.
4. Dururira lotion mumidziyo yakaoma uye gadzira pa 110 degrees Celsius kwemaawa 1 kusvika ku2.Usambofa wakabika pane kupisa kwakanyanya.
5. Kutonhorera uye chengetedza.

Sarudzo yechipiri:

1. Shamba nemvura yepombi kakawanda
2. Iise mubeaker izere nemvura yakachena (Millipore pure water machine) uye sonicate kwemaminitsi gumi nemashanu.
3. Shandura mvura uye ultrasound kwemaminitsi gumi nemashanu
4. Nyura mubeaker yakazadzwa neabsolute ethanol (Sinopharm Group, Analytical Pure)
5. Pakupedzisira, bvisa uye uite kuti mhepo iome.

Sarudzo yechitatu:

1. Kutanga kunyura mu methanol (chromatographically pure), uye ultrasonically yakachena kwemaminitsi makumi maviri, wozodira methanol yakaoma.
2. Zadza mabhodhoro emuenzaniso nemvura, uye ultrasonically yakachena kwemaminitsi makumi maviri, dururira mvura .
3. Pukuta mabhodhoro emuenzaniso mushure.

Sarudzo Yechina:

Nzira yekugezesa yemuenzaniso mabhodhoro yakafanana nekugadzirira kwechikamu chemvura, etc. Kutanga, shandisa doro rezvokurapa kuti unyorove kwemaawa anopfuura mana, ipapo ultrasound kwehafu yeawa, wozodurura doro rezvokurapa, uye shandisa mvura. kwehafu ye ultrasound.Maawa, simbura nemvura uye woomesa.

Sarudzo yechishanu:

Kutanga, nyura mune yakasimba oxidizing yekuchenesa mhinduro (potassium dichromate) kwemaawa makumi maviri nemana, uye ipapo shandisa mvura yakasvibiswa mu ultrasonic Geza iyo katatu pasi pemamiriro ezvinhu, uye pakupedzisira uigeze ne methanol kamwe chete, wozoiomesa kuti ishandiswe.
Iyo caps septas inofanirwa kutsiviwa, kunyanya kana uchiongorora masara emishonga yezvipembenene, zvikasadaro zvinokanganisa huwandu hwemhedzisiro.
Asi kana mamiriro ezvinhu achibvumidza, edza kushandisa zvinoraswa, senge zvinoraswa PTFE ekuisa kana epamba epurasitiki inoisa (inenge 0.1 yuan/chidimbu), uye sampuli mabhodhoro zvakanaka.Kudzokorora kushandiswa uye hazvidi kucheneswa.

Sarudzo yechitanhatu:

(1) kuomesa kuchenesa maitiro nemhedzisiro inoshanda:
No1.Mushure mekunge mabhodhoro ekuenzanisira ashandiswa, simbura mabhodhoro emuenzaniso nemvura inoyerera kutanga, uye suka samuenzaniso yasara (unogona kuizunza noruoko panguva imwe chete);
No2, wobva waisa mabhodhoro emuenzaniso mu potassium dichromate washing liquid bubble, uye painoungana Paunosvika pane imwe nhanho kana kuti paunenge uri mumamiriro ezvinhu akanaka, bvisa kubva mulotion tank woiisa musefa yepurasitiki yekicheni. use.Sukurudza zvakakwana nemvura yepombi.Iwe unogona kudzokorodza sefa uye kuzunungusa pakati;
No3.Shandisa mvura yepombi kuchenesa ultrasonically katatu mushure mekugeza.Kutenderedza, zviri nani kuzunungusa kunze kwemvura mune yemuenzaniso mabhodhoro mushure mega yega ultrasonic kuchenesa;
No4, zvino shandisa katatu distilled mvura (kana kunatswa mvura, deionized mvura) ne 1.3 ultrasonic kuchenesa katatu;
No5, wobva washandisa chromatographic pure methanol ultrasonic kuchenesa 2-3 nguva, zviri nani zvakare
zunza methanol kubva mumidziyo yemuenzaniso mushure mekuchenesa kwega kwega;
No6.Isa mabhodhoro emuenzaniso muchoto uye woomesa paanenge 80 degrees, uye inogona kushandiswa.

(2) sampuli mbiya dzakatengwa kumaka nemavara akasiyana:

kana waona kuti pane diki diki mucherechedzo mucherechedzo mbiya, iyo isiri yekunaka, asi ine mashandisiro ayo.Paunenge uchitenga, zviri nani kutenga zvivharo zvakawanda zvemavara akasiyana.

Semuenzaniso: rabhoritari yako inovhura mapurojekiti maviri A neB panguva imwe chete.Kekutanga A purojekiti inoshandisa chena sampuli mabhodhoro, uye B chirongwa chinoshandisa yebhuruu sampuli mabhodhoro.Mushure mekunge bvunzo yapera, inocheneswa zvinoenderana neiyo nzira iri pamusoro, uye yechipiri kuyedza Panguva iyoyo, shandisa bhuruu sampuli mabhodhoro eA purojekiti, machena emuenzaniso mabhodhoro eB purojekiti, zvichingodaro, izvo zvinogona kunyatso kudzivirira dambudziko rinokonzerwa ne kusvibiswa kubasa rako.

Nyora panoperera

1. Mainjiniya akati wandei akataura kuti: Shandisa mufuro wevira pa400 madhigirii kubheka kwehafu yeawa, zvinhu zveorganic zvaenda;
2. Isai mabhodhoro emuenzaniso muchoto chemuffle kuti chiomeswe pa 300 degrees Celsius.Injiniya yeAgilent kubva kuBeijing akati paakauya kuchoto chemuffle, bvunzo yacho haizove ruzha mushure mekubikira muchoto chemoto pa300 degrees kwemaawa matanhatu.

zvakare………..
Madiki volumetric flasks, maflasks akaita pear evaporation, uye mamwe magirazi ekuongorora kana pretreatment anogona kucheneswa nekureva nzira iyi.

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Nguva yekutumira: Feb-25-2022